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Melatonin and BMP6/Smad1/5/9 signaling both regulated endothelial regeneration post-OGD A Representative photographs of Western blot of VEGF, Ang1, BMP6, p-Smad1/5/9, Smad1/5/9 and GAPDH protein expression in ECs at 9 h after OGD. B - E Quantification of relative VEGF, Ang1, BMP6, p-Smad1/5/9/Smad1/5/9 protein expression at 9 h after OGD (* P < 0.05, ** P < 0.01, *** P < 0.001; n = 6 each group). F - K Quantification of relative VEGF, Ang1, BMP6, Smad1, Smad5, and Smad9 mRNA expression at 9 h after OGD (* P < 0.05, ** P < 0.01, *** P < 0.001; n = 6 each group)

Journal: Molecular Neurobiology

Article Title: Melatonin Promotes Cerebral Angiogenesis in Ischemic Mice via BMP6/Smad1/5/9 Pathway

doi: 10.1007/s12035-025-04969-4

Figure Lengend Snippet: Melatonin and BMP6/Smad1/5/9 signaling both regulated endothelial regeneration post-OGD A Representative photographs of Western blot of VEGF, Ang1, BMP6, p-Smad1/5/9, Smad1/5/9 and GAPDH protein expression in ECs at 9 h after OGD. B - E Quantification of relative VEGF, Ang1, BMP6, p-Smad1/5/9/Smad1/5/9 protein expression at 9 h after OGD (* P < 0.05, ** P < 0.01, *** P < 0.001; n = 6 each group). F - K Quantification of relative VEGF, Ang1, BMP6, Smad1, Smad5, and Smad9 mRNA expression at 9 h after OGD (* P < 0.05, ** P < 0.01, *** P < 0.001; n = 6 each group)

Article Snippet: The primary antibodies used were rat anti-CD31 (1:50, BD Biosciences Cat# 550274, RRID: AB_393571) to label endothelial cell (ECs) and rabbit anti-glial fibrillary acidic protein (GFAP, 1:200, BOSTER Cat# BM4287) to label astrocytes.

Techniques: Western Blot, Expressing